Journal: Viruses

Article Title: Monocyte Dynamics in Chikungunya Fever: Sustained Activation and Vascular-Coagulation Pathway Involvement

doi: 10.3390/v17091224

Figure Lengend Snippet: Circulating markers of inflammation and vascular dysfunction. ( A – D ) Circulating levels of IL-6, TNF-α, IL-17A, and IL-10 in patients with CF during the acute (n = 46), post-acute (n = 4), chronic (n = 8) phases of infection, and in HD (n = 10). ( E , F ) Circulating levels of TF and TFPI in CF patients during the acute (n = 37), post-acute (n = 4), and chronic (n = 8) phases, and in HD (n = 10). ( G ) Circulating levels of VEGF in CF patients during the acute (n = 53), post-acute (n = 4) and chronic phases (n = 8), and in HD (n = 10). ( H ) Circulating levels of EGF in CF patients during the acute (n = 39), post-acute (n = 4), chronic phases (n = 8), and in HD (n = 10). ( I ) Circulating levels of PDGF-BB in CF patients during the acute (n = 43), post-acute (n = 4), chronic phases (n = 8), and in HD (n = 10). Data are presented as medians with interquartile ranges (IQR). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA followed by Kruskal–Wallis test and Dunn’s multiple comparisons test and using the Mann–Whitney test.

Article Snippet: Circulating levels of TF and TFPI in HD and CHIKV-infected patients were measured using the Human Coagulation Factor II/Tissue Factor ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the Human TFPI ELISA Kit (R&D Systems, Minneapolis, MN, USA), following the manufacturer’s instructions.

Techniques: Infection, MANN-WHITNEY

Journal: Thrombosis and Haemostasis

Article Title: NF-κB-Mediated Upregulation of Tissue Factor Contributes to the Procoagulant Phenotype of Smooth Muscle Cells from Abdominal Aorta Aneurysm in Human

doi: 10.1055/a-2665-2510

Figure Lengend Snippet: Expression of tissue factor and anticoagulant proteins in AAA. ( A ) Tissue factor (TF) synthesis by SMCs cultured from HA and AAA aortas ( n = 9–13). ( B ) TF antigen in SMCs ( n = 10). ( C ) TF activity at the surface of SMCs ( n = 8). ( D ) TF activity in tissue-conditioned media from HA and AAA ( n = 10). ( E ) Tissue factor pathway inhibitor (TFPI) mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8–10). ( F ) TFPI protein in SMCs ( n = 14–16). ( G ) TFPI protein in cell supernatants ( n = 12–15). ( H ) Thrombomodulin (TM) mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8–10). ( I ) TM protein in SMCs ( n = 11–12). ( J ) TM protein in cell supernatants ( n = 12–14). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.

Article Snippet: TF pathway inhibitor (TFPI) was measured in cell culture lysates and supernatants using the Asserachrom total TFPI assay (Diagnostica Stago, Asnières sur Seine, France).

Techniques: Expressing, Cell Culture, Activity Assay, MANN-WHITNEY